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mcd99 991 Followers, 486 Following, 146 Posts - Riley McDaniel (@riley_mcd99) on : Wife | Mom to be School. Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ balanced salt solution (HBSS). Cells were washed twice in phosphate-bufered sa

mcd99 login, Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40.